• br Discussion In this study we obtained

  2018-10-20

  

  Discussion In this study, we obtained proof-of-concept data to support our hypothesis that AM can rescue OPC differentiation into mature oligodendrocytes under pathological conditions. Our pharmacological approaches also showed that the AM receptor and PI3K/Akt would mediate these AM effects. AM

• We developed our novel targeting technique in order to overc

  2018-10-20

  

  We developed our novel targeting technique in order to overcome one of the major problems with stem cell therapy, namely the high wash-out of injected stem EZ Cap Reagent AG (3\' OMe) by the blood and low engraftment rate. This high-washout of over 90% by the blood is irrespectively of the delivery

• The original XO karyotype Fig of the donor CVCs

  2018-10-20

  

  The original 45 XO karyotype (Fig. 1) of the donor CVCs was maintained in TS ViPSCs even after prolonged maintenance (passage 25) indicating that the process of reprogramming with episomal vectors and in vitro culture conditions did not alter this iPSC line chromosomally. The iPSC line established i

• The in vitro production and expression of

  2018-10-20

  

  The in vitro production and expression of ECM proteins collagens I and IV and laminin differed between the perivascular derivatives. In the body, pericytes produce ECM in the subendothelial basement membrane of capillaries, while both vSMCs and pericytes produce ECM in the tunica media layer of larg

• Our studies show that only a subpopulation of hESCs

  2018-10-20

  

  Our studies show that only a subpopulation of hESCs at the top of the hierarchy possesses an extensive capacity for self-renewal. Polanco et al. recently showed that metaformin in this compartment could reestablish the continuum of cell states seen in unfractionated cultures (Polanco et al., 2013).

• MSCs can express several WNT ligands Cho et al

  2018-10-20

  

  MSCs can express several WNT ligands (Cho et al., 2006; Etheridge et al., 2004). This endogenous WNT signaling may play a negligible role in MSC expansion, because we found no significant effects on cell proliferation or subsequent differentiation when endogenous WNT proteins were prevented. However

• br Experimental Procedures br Author Contributions br Acknow

  2018-10-20

  

  Experimental Procedures Author Contributions Acknowledgments Introduction With the first reports on generating human induced pluripotent stem Teneligliptin hydrobromide (hiPSCs) from human cells (Takahashi et al., 2007; Yu et al., 2007), the controversy regarding the ethics of research

• Differentiating PGCLCs in aggregates from i cultured human

  2018-10-20

  

  Differentiating PGCLCs in XMD8-92 Supplier from 4i cultured human pluripotent stem cells initially utilized a 48-h pre-induction step on Vitronectin/Gelatin coated plates in media composed of bFGF and either TGFβ1 or ACTIVIN A. However subsequent experiments showed that the pre-induction step befor

• Our selection free targeting approach enables rapid generati

  2018-10-20

  

  Our selection-free targeting approach enables rapid generation of knockin reporter lines, though it also requires either using established iCas9 MC 1568 manufacturer or creating new iCas9 lines in a desired hPSC background. The upfront effort for generating iCas9 cells is relatively small due to th

• To gain greater insight into the

  2018-10-20

  

  To gain greater insight into the broad applicability for cell delivery, HAMC was investigated for the delivery of adult mouse NSCs (Morshead et al., 1994) to the brain. Significantly more cells were observed when delivered in HAMC versus artificial cerebrospinal fluid (aCSF) controls. Moreover, the

• The Gata site regulates a large

  2018-10-20

  

  The Gata2 +9.5 site regulates a large gene cohort in hemogenic endothelium, and these genes do not parse into a single pathway (Gao et al., 2013). The genes include Runx1, Lyl1, and Mpl, positive mediators of HSC generation and/or function. The identification of vital constituents of the +9.5-depend

• VER 155008 br Experimental Procedures br Author Contribution

  2018-10-20

  

  Experimental Procedures Author Contributions Acknowledgments We thank Y. Sasai (RIKEN, Japan) for the RAX-GFP mESC line, M. Wegner (University of Erlangen, Germany) for SOX9, E. Tanaka (CRTD, Germany) for RAX and CRX, and D. Forrest (NIDDK, USA) for TRβ2 antibodies. We thank J. Brzezinski (

• Forces generated by actomyosin networks within cells and for

  2018-10-20

  

  Forces generated by actomyosin networks within ap-1 transcription factor and forces transmitted through intercellular adhesions are coordinated in driving self-organized tissue morphogenesis (Heisenberg and Bellaiche, 2013). We determine that retinal organoid morphogenesis is mediated by ROCK-regula

• br Experimental Procedures br Author

  2018-10-20

  

  Experimental Procedures Author Contributions Acknowledgments We wish to thank Nicole Varain and Donna Colon for their excellent technical support assisting with the fetal sheep transplants. This work was supported by NIH, NHLBIR01HL097623, and the Intramural Pilot Program of Wake Forest Sch

• br Results br Discussion The MSC secretome

  2018-10-20

  

  Results Discussion The MSC secretome is being explored in pre-clinical and clinical trials as one of the primary mediators of cardiovascular repair via immunomodulatory effects (Ranganath et al., 2012). However, to date, approaches have not met with success as initially hoped. The immunomodula

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