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Primases are classified into two major
2020-11-28
Primases are classified into two major groups: first, the DnaG primases, found in bacteria and bacteriophages, and second, the archaeoeukaryotic primases. Remarkably, bacterial and archaeoeukaryotic primases have no structural similarity and, presumably, evolved independently (Leipe et al., 1999). T
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In the present study synthesis of Bi MoO nanoplates via
2020-11-28
In the present study, synthesis of Bi2MoO6 nanoplates via DNA-templated hydrothermal method has been undertaken. The synthesis was done through the hydrothermal method by means of the reaction of bismuth nitrate pentahydrate (Bi(NO3)3·5H2O) with sodium molybdate dihydrate (Na2MoO4·2H2O) precursor in
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To determine whether the same concentration of humic acid ca
2020-11-28
To determine whether the same concentration of humic Caspase-6 Colorimetric Assay Kit causes changes in PCR efficiency, we performed another experiment in which the humic acid was added to the PCR mix after bisulfite conversion. All samples that were amplified in the presence of humic acid (thick
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br Biological roles of DGK br
2020-11-28
Biological roles of DGKϵ Summary and future perspectives The most Benzethonium Chloride segment of DGKϵ, comprised of residues 20–42, appears to have no role in binding the lipid substrate DAG or in the acyl chain specificity of substrate phosphorylation. Nevertheless, the sequence in this se
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Possibly there are similarities among euphorb plant species
2020-11-28
Possibly there are similarities among euphorb plant species but extraction of the 7ACC2 receptor present in the microsomal fraction is needed for testing their preferences for substrates. Although R. communis, E. lathyris and E. lagascae belong to the same family, the DGAT activity of their microso
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br NADH and NADPH Turnover
2020-11-28
NADH and NADPH Turnover and the Putative Role of Alternative NAD(P)H Dehydrogenases Still very little is known about the metabolic function of external and internal alternative NADH:UQ oxidoreductases, and almost nothing is known about the mechanisms underlying their metabolic regulation, especia
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Introduction Tumor necrosis factor TNF is a pro inflammatory
2020-11-28
Introduction Tumor necrosis factor (TNF) is a pro-inflammatory mediator with the capacity to induce apoptosis (Benderska et al., 2012). Recent reports have shown that TNF might trigger cell death, at least in part, by directly affecting the reorganization of the cgrp antagonist cytoskeleton (Campos
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br Materials and methods br Results
2020-11-28
Materials and methods Results Discussion Our study assessed the cytokine and chemokine changes associated with first onset PP as compared to HP and HNP groups. In the monocytes/ macrophages/ dendritic 96 7 panel, IL-6 levels were found to be significantly elevated in both HP and PP groups w
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Interestingly some drugs have been
2020-11-28
Interestingly, some drugs have been shown to be effective in the regulation of cytokine production (such as IL-2, IL-10, IL-27, IL-35, IL-37 and transforming growth factor-β, TGF-β) and, therefore, having a key role in the management of certain inflammatory-based clinical conditions (Banchereau et a
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Moreover a ligand for fusin has recently been found the
2020-11-28
Moreover, a ligand for fusin has recently been found: the lymphocyte chemoattractant stromal-derived factor-1 (SDF-1) binds to CHO Pam3CSK4 Biotin transfected with fusin and promotes secondary messenger production (Bleul et al., 1996; Oberlin et al., 1996). A new name, CXCR-4, has been proposed for
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3254 Immunofluorescence Cells were fixed with paraformaldehy
2020-11-28
Immunofluorescence. Cells were fixed with 4% paraformaldehyde (PFA) in PBS and extracted by 0.5% Triton X-100-PBS, or fixed in cold methanol. The cells were immunostained with mouse monoclonal 3254 against α-tubulin (Sigma), γ-tubulin (Sigma), Ran (Upstate), rabbit polyclonal antibodies against per
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Increasing age among middle aged subjects years old
2020-11-27
Increasing age among middle-aged subjects (30–59years old) was associated with higher levels of cholesterol synthesis markers (although without statistical significance) and also of cholesterol Cy5 EGFP australia markers (with statistical significance for campesterol and sitosterol); this finding is
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Mouse FGF21 / Fibroblast Growth Factor 21 Protein (His Tag)
2020-11-27
Discussion Of the age-related differences in serum non-cholesterol sterols, we first deal with the cholesterol precursors. After birth, the markedly elevated serum desmosterol values decreased during the first year of life, and after 1 year of age they were similar between the other age groups. S
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br Acknowledgements br Introduction Water and
2020-11-27
Acknowledgements Introduction Water and electrolytes constitute an integral part of all living things, and Caspase-10/b, human recombinant protein governing their movements play important roles in a wide variety of physiological and/or pathophysiological processes. Given the importance of ch
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AON successfully alleviated the myotonic phenotype
2020-11-27
AON successfully alleviated the myotonic phenotype in DM model mice [18], [23]. To screen for an optimal AON sequence, we used 25-mer phosphorothioate 2′ O-methyl RNA molecules that covered the exon 6B or exon 7A. Unexpectedly, AON 7A (+63−8) which covers the boundary of intron 7A and exon 7A enhan
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